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    SAFETY DATA SHEET Page: 1 of 6 Copper Sulfate Solution .

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    Identification and functional . - BMC Plant Biology

    CBL1 is a calcium sensor that regulates drought, cold and salt signals in Arabidopsis. Overexpression of CBL1 gene in Arabidopsis and in Ammopiptanthus mongolicus showed different tolerant activities. We are interested in understanding the molecular mechanism of the upstream region of the CBL1 gene of A. mongolicus (AmCBL1). We investigated and characterized the promoter of the AmCBL1 gene .

    Isopropyl β-D-1-thiogalactopyranoside - Wikipedia

    Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent.This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.. IPTG, unlike allolactose, is not hydrolyzable by β-galactosidase.

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    Rapid validation of transcriptional enhancers using .

    Schematic representation of the enhancer validation pipeline using a luciferase-based transient assay. a Candidate enhancers were predicted based on DHSs and other chromatin datasets.b The predicted enhancer sequence was synthesized and cloned in the pCAMBIA-CRE-LUC vector containing a mini 35S promoter and the firefly luciferase reporter gene, and was transferred into Agrobacterium strain …

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    Choksi Labs >> Shareholding Pattern - June 2019

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    Vector Detail - Arabidopsis

    ABRC Stock Donation; External Links; Gene Class Symbol Registration; Gene Family Data; Online Submission for Authors and Others; Gene Structure Additions/Modifications; Marker and Polymorphism Data; Metabolic Pathway Data; Phenotypes; Protocols; 2010 Functional Genomics Gene List; News. TAIR News; Arabidopsis Newsgroup; TAIR Newsletter Archive .

    Edit-R All-in-one Lentiviral sgRNA Non-targeting Controls

    In the Edit-R All-in-one Lentiviral sgRNA vector backbone, the gene-specific guide RNA is expressed under the control of a human U6 promoter, while expression of the Cas9 and puromycin resistance marker (Puro R) is driven from either the human EF1α or the mouse CMV promoter.The plasmid contains the Amp R resistance marker for growth and selection in E. coli.

    Engineering of synthetic, stress-responsive yeast promoters

    Ultimately, we selected the YGP1 promoter (YGP1pr) as a candidate promoter to engineer based on the aforementioned criteria.Ygp1p is a glycosylated, secreted cell wall-associated protein, expressed during conditions of stress or nutrient depletion (41,42), but which has also been reported to be induced by low pH across different strains and .

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    Quantitative comparison of expression with adeno .

    Sep 11, 2001· This study compared a range of mammalian CNS expression cassettes in recombinant adeno-associated virus (AAV-2) vectors using strong endogenous promoter sequences, with or without a …

    MS 275 (CAS 209783-80-2): R&D Systems

    Biological Activity Class I HDAC inhibitor (IC 50 values are 0.18, 0.74, 44.9 and >100 μM for HDAC1, 3, 8 and 6, respectively). Exhibits antiproliferative effects and induces apoptosis in a range of tumor cell lines in vitro and in vivo.Increases estrogen receptor α- and aromatase expression in breast cancer cells.

    Large scale validation of an efficient CRISPR/Cas-based .

    Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli. . The cas9 gene and the tracrRNA coding sequence are under the control of constitutive promoters while the λ Red gene cassette is transcribed from the pBAD . Stock preparations of strains were prepared in LB + 20% glycerol and stored at .

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    Hybrid phenolic-inducible promoters towards construction .

    Jun 28, 2018· A hybrid promoter engineering approach was utilized for the construction of phenolic-inducible promoters of higher strength. The hybrid promoters were constructed by replacing the spacer region of an endogenous promoter, PemrR present in E. coli that was naturally inducible by phenolics.

    N,N'-Diethylthiourea 105-55-5 | TCI EUROPE N.V.

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    CAS No. 105-55-5 | Sigma-Aldrich

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    N,N'-diethylthiourea - CAS # 105-55-5 Information provided on N,N'-diethylthiourea (105-55-5) is for reference only and is subject to change. There is no warranty of accuracy or completeness of any information contained herein.

    A functional analysis of the CREB signaling pathway using .

    Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding .

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    024858 - STOCK Gt(ROSA)26Sor<tm1.1(CAG-cas9*,-EGFP)Fezh>/J

    These CRISPR/Cas9 knockin mice constitutively express CRISPR associated protein 9 (cas9) endonuclease, a 3X-FLAG epitope tag and EGFP in a widespread fashion under the direction of a CAG promoter. Breeding to a germ-line expressed Cre strain during development removed a Lox-Stop-Lox (LSL) sequence to allow ubiquitous expression of the knockin construct.

    Repressible promoters – A novel tool to generate .

    Repressible promoters are a useful tool for down-regulating the expression of genes, especially those that affect cell viability, in order to study cell physiology. They are also popular in biotechnological processes, like heterologous protein production. Here we present five novel repressible Pichia pastoris promoters of different strength: P SER1, P MET3, P THR1, P PIS1 and P THI11 . eGFP .

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